visium hd spatial transcriptome analysis  (10X Genomics)

Journal: Pharmacological research

Article Title: Neuroprotectin D1 and GPR37 protect against chemotherapy-induced peripheral neuropathy and the transition from acute to chronic pain.

doi: 10.1016/j.phrs.2025.107746

Figure Lengend Snippet: Fig. 8. Spatial transcriptomics analysis of GPR37 expression in human DRG. (A) Schematic diagram of flowchart for spatial transcriptomics analysis in human DRG using 10x Visium HD platform and the gene expression matrices for the 8×8 μm bins. (B) Colocalization of GPR37 with CALCA in peptidergic neuron (indicated by filled arrows). Scale bar, 20 µm. The filled arrow with a cross indicates a bin with GPR37 expression only. (C) Co-expression of GPR37 and CSF1R in macrophages (indicated by an open arrow). Scale bar, 20 µm. The filled arrow with a cross indicates GPR37 expression in neuron. (D) Co-expression of GPR37 and CD163+ in M2 macrophage (indicated with open arrows). Scale bar, 20 µm. Left panels in B-D are H&E images showing the morphology of human DRG neurons, surrounded by many non-neuronal cells.

Article Snippet: Visium HD Data Analysis: Space Ranger software (v3.1.2 10x Genomics) was used to process raw FASTQ files, align the reads to the reference transcriptome, and generate gene expression matrices for the default 2um, 8um, and 16um binning in each sample.

Techniques: Expressing, Gene Expression